Oral Biology: Molecular Techniques and Applications
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Part I: Saliva and Other Oral Fluids
1. RNA Sequencing Analysis of Saliva exRNA
Karolina Elżbieta Kaczor-Urbanowicz and David T.W. Wong
2. Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry
Nagihan Bostanci and Kai Bao
3. Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics
Pingping Han, Xiang Li, Wei Wei, and Saso Ivanovski
4. Antioxidant Micronutrients and Oxidative Stress Biomarkers
Irundika H.K. Dias, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Iain L.C. Chapple, and Melissa M. Grant
Part II: Molecular Biosciences
5. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings
Jos?F. Siqueira Jr and Isabela N. R鐋as
6. The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes
Nicholas C.K. Heng and Jo-Ann L. Stanton
7. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
Jos?F. Siqueira Jr, Mitsuo Sakamoto, and Alexandre S. Rosado
8. Bioinformatic Approaches for Describing the Oral Microbiota
Kristi Biswas, Michael W. Taylor, and David T.J. Broderick
9. Adhesion of Yeast and Bacteria to Oral Surfaces
Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, and Ann R. Holmes
10. Quantitative Analysis of Periodontal Pathogens Using Real Time Polymerase Chain Reaction (PCR)
Ma Jos?Marin, Elena Figuero, David Herrera, and Mariano Sanz
11. Methods to Study Antagonistic Activities Among Oral Bacteria
Fengxia Qi and Jens Kreth
12. Generation of Multi-Species Oral Bacteria Biofilm Models
Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston, and Gordon Ramage
13. Markerless Genome Editing in Competent Streptococci
Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. 囈dal, Fernanda C. Petersen, and Donald A. Morrison
14. A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System
Sven E. Niklander and Keith D. Hunter
15. Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients
Sai Shyam Vasantharajan, Edward Barnett, Elin S. Gray, Euan J. Rodger, Michael R. Eccles, Sharon Pattison, Fran Munro, and Aniruddha Chatterjee
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