A Laboratory Guide to the Mammalian Embryo

Drawing from recent work with embryos from rodents, domestic animals, and primates, this practical guide describes the theory and protocols for performing biochemical, microscopic, and genetic techniques that have been developed and applied at the single-cell level. The techniques are the result of both new research and updates to established procedures. Topics of the 24 contributions include micromanipulation of gametes, assessment of preimplantation embryo viability, metabolic pathway activity, relative mRNA transcript abundance analysis by RT-PCR, 2D protein gel database analysis of oocytes, and somatic cell nuclear transfer. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)