醫學生物學實驗（簡體書）

《醫學生物學實驗(英文版)》內容簡介：This manual is designed to be used in a course of experiments in medical biology. It will serve mainly the needs of international students of clinical medical major. It will serve any student who wishes to leam both major principles and modern techniques of medical biology.
By using this manual,students will develop laboratory skills,become familiar with the methods of science,and learn important concepts of experiments in medical biology. The manual provides students with an opportunity to apply what they have learned in textbook and lectures in an interesting and informative way.

《醫學生物學實驗(英文版)》是醫學教育改革系列教材之一。

Chapter1 Introduction to the Medical Biology Laboratory
11 Objectives of a Teaching Medical Biology Laboratory
12 Laboratory Rules
13 Laboratory Safety
14 Common Laboratory Equipments
15 Experimental Methods in Medical Biology
16 Evaluation of Experimental Data and Laboratory Reports

Chapter 2 Experiments in Medical Biology
Experiment 1 The Use of Glass Pipets and Pipettors N
Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins
Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie BriHiant Blue As Tay NN
Experunent 4Quantitative Determination of Proteins by Folin-phenol Assay
Experiment 5 Determination oF Proteins by UV Absorption
Experiment 6 Separation of Arrmio Acid MixtIHes by Ion-exchange Chromatography
Experiment 7 Separation of Serum y-globulins by Ion-exchange Chromatography
Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane
Electrophoresis
Experiment 9 Separation and Purity Determination of Serum y-globulins by SDS-polyacrylamide Gel Electrophoresis
Experiment 10 Determination of Serum Glutamicpyruvic Transaminase Activity
Experiment 11 Determination of Serum Urea Nitrogen Content
Experiment 12 Determination of Blood Glucose Concentration
Experiment 13 Subculture of Adhesion Cells NN
Experiment 14 Counting and Determination of Live/Dead Cells
Experiment 15 Preparation of Cell Samples for Observation of Cell Morphologies
Experiment 16 Mitosis and Meiosis of Cells
Experiment 17 Intracellular Distribution of Biomacromolecules
Experiment 18 Isolation of Subcellular Components
Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells
Experiment 20 Banding Pattern of Human Chromosomes
Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells
Experiment 22 Isolation,Amplification and Identification of the pactin Gene Fragment from Mouse Liver Cells
Experiment 23 Purification of Target DNA and Generation of Recombinant DNA
Experiment 24 Transformation and Screening of Recombinant DNA
Experiment 25 Determination of Serum Proteins Using Western Blotting

The formation of G banding is closely related to the characteristics of Giemsa stain,which is a mixture of methylene blue,azure, and eosin. Bothmethylene and azure belong to the thiazine dyes and they bind to the phosphate groups in DNA and do not bind to proteins. As a result,chromosomestaining is determined by the binding of two thiazine molecules to DNA. On top of that,one molecule of eosin binds and forms the thiazineeosin complexat the ratio of 2: 1. Besides,staining is also enhanced by the hydrophobicenvironment which favors dye complex accumulation. Chromosomes rich in hydrophobic motifs facilitate the formation of the thiazineeosin complex. This indicates that proteins are important factors for G banding. In the repeated DNA sequences in chromosomes,the transcriptional activity is low,and packaging proteins are stable. Those proteins can form hydrophobicahelix motif with strong disulfide bonds, thus facilitate the accumulation of the dye complex, showing dark bands under the microscope.On the other hand,DNA regions encoding structural genes-have high transcriptional activity,and the packaging proteins are loosely bound.