Recombinant Proteins in Plants: Methods and Protocols

1. Recombinant Protein Production in Plants - A Brief Overview of Strengths and Challenges

Stefan Schillberg and Holger Spiegel

Part I: Plant Production Systems and Applications

2. Production of Recombinant Proteins in Transgenic Tobacco Plants

Tim H. Szeto, Pascal M.W. Drake, Audrey Y.-H. Teh, Nicole Falci Finardi, Ashleigh G. Clegg, Mathew J. Paul, Rajko Reljic, and Julian K.-C. Ma

3. Molecular Farming in Seed Crops: Gene Transfer into Barley (Hordeum vulgare) and wheat (Triticum aestivum)

Eszter Kapusi and Eva Stoger

4. Cell Biology Methods to Study Recombinant Proteins in Seeds

Elsa Arcal疄, Emanuela Pedrazzini, Ulrike H顤mann-Dietrich, Alessandro Vitale, and Eva Stoger

5. Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells

Catherine Navarre and Fran蔞is Chaumont

6. Production of Recombinant Proteins by Agrobacterium-mediated Transient Expression

Holger Spiegel, Stefan Schillberg, and Greta N闤ke

7. Specific packaging of custom RNA molecules into Cowpea Mosaic Virus-Like Particles

Hadrien Peyret and George P. Lomonossoff

8. Plant-based Cell-Free In Vitro Transcription and Translation of Recombinant Proteins

Matthias Buntru, Simon Vogel, Ricarda Finnern, and Stefan Schillberg

Part II: Downstream Processing

9. Strategies for Efficient and Sustainable Protein Extraction and Purification from Plant Tissues

Johannes F. Buyel

10. Improving Recombinant Protein Recovery from Plant Tissue Using Heat Precipitation

Holger Spiegel

11. Techno-economic Modeling and Simulation for Plant-Based Manufacturing of Recombinant Proteins

Matthew J. McNulty, Somen Nandi, and Karen A. McDonald

Part III: Optimization Strategies

12. Optimization of Vectors and Targeting Strategies Including Goldenbraid and Genome Editing Tools -

GoldenBraid assembly of multiplex CRISPR/Cas12a guide RNAs for Gene Editing in Nicotiana benthamiana

Beatriz Gonz嫮ez, Marta Vazquez-Vilar, Javier S嫕chez-Vicente, and Diego Orz墈z

13. Advanced Fusion Strategies for The Production Of Functionalized Potato Virus X virions

Christina Dickmeis and Ulrich Commandeur

14. Knockout of Glycosyltransferases in Nicotiana benthamiana by Genome Editing to Improve Glycosylation of Plant-Produced Proteins

Julia Jansing and Luisa Bortesi

15. A bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in planta

Philippe V. Jutras, Isobel Dodds, and Renier A. L. van der Hoorn

16. Statistical Designs to Improve Downstream Processing

Johannes F. Buyel

Part IV: Regulatory Issues

17. Regulation of Molecular Farming Products

Penny AC Hundleby, Marc-Andr?D'Aoust, Carolyn Finkle, Judith Atkins, and Richard M. Twyman

18. Freedom to Operate Analysis of Molecular Farming Projects

Harry Thangaraj