Lipid-Mediated Signaling

As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade’s worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.
Part I describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated signaling. Part II highlights the use of mass spectrometry to ascertain the lipid content in various systems. Part III contains two chapters devoted to techniques used to determine the role of lipids in the activation of gene transcription. Part IV rounds out the book’s solid coverage by focusing on methods used to assess fatty acid uptake and metabolism.
Lipid-Mediating Signaling is an unrivaled reference for this rapidly expanding, yet resource-limited field, and it is a valuable resource for for cell biologists, biochemists, and pharmacologists, as well as for researchers studying signaling transduction.

Eric J. Murphy, University of North Dakota, Grand Forks, USAThad A. Rosenberger, University of North Dakota, Grand Forks, USA

ENZYME SYSTEMS INVOLVED IN LIPID-MEDIATED SIGNALINGMethods for Measuring the Activity and Expression of Phospholipases A2 Prerna Rastogi, Janhavi Sharma, and Jane McHowatMethods of Measuring the Activity and Expression of Phospholipases C Ryan A. Frieler, John A. Watt, and Thad A. RosenbergerMethods for Measuring the Activity and Expression of Phospholipase DWenjuan Su and Michael A. FrohmanMethods for Measuring the Activity and Expression of Diacylglycerol Kinase Kaoru Goto, Yasukazu Hozumi, Tomoyuki Nakano, Alberto M. Martelli, and Hisatake KondoMethods for Measuring Endocannabinoid Production and Expression and Activity of Enzymes Involved in the Endocannabinoid System Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di MarzoMASS SPECTROMETRY METHODS FOR LIPID ANALYSISMass Spectrometry Methods for the Analysis of Phospholipid Molecular Species Analysis: A Shotgun Lipidomics ApproachXianlin Han, Hua Cheng, Xuntian Jiang, and Youchun ZengMass Spectrometry Methods for the Analysis of Bioactive Sphingolipids: A High-Performance Liquid Chromatography/Tandem Mass Spectrometry Approach Jacek Bielawski, Motohiro Tani, and Yusuf A. HannunMass Spectrometry Methods for the Analysis of Oxidant Stress in Biological Fluids and Tissues: Quantification of F2-Isoprostanes and F4-Neuroprostanes Using Mass Spectrometry Ginger L. Milne and Jason D. MorrowLIPID-MEDIATED REGULATION OF GENE EXPRESSIONMethods for Assessing the Role of Lipid-Activated Nuclear Receptors in the Regulation of Gene Transcription Jenny Kaeding, Jocelyn Trottier, and Olivier BarbierFluorescence Methods to Assess the Impact of Lipid Binding Proteins on Ligand-Mediated Activation of Gene ExpressionHeather A. Hostetler, Avery L. McIntosh, Anca D. Petrescu, Huan Huang, Barbara P. Atshaves, Eric J. Murphy, Ann B. Kier, and Friedhelm SchroederMETHODS TO ASSESS FATTY ACID AND PHOSPHOLIPID METABOLISMMethods for Measuring Fatty Acids Using Chromatographic Techniques Gwendolyn Barceló-Coblijn, Cameron C. Murphy, and Eric J. MurphyMethods for Measuring Fatty Acid Uptake and Targeting in Cultured Cells and In VivoPaul G. Millner, Serena M. Lackman, and Eric J. MurphyMethods for Kinetic Analysis of Fatty Acid Incorporation and Turnover In Vivo: A Steady-State Kinetic Radiotracer Approach Dhaval P. Bhatt and Thad A. Rosenberger