Transcriptional Regulation in Eukaryotes ─ Concepts, Strategies, and Techniques

In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. The first edition of this book was a comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Written by two experienced investigators and based in part on the Gene Expression course taught at CSH, the book directly addressed all the concerns of a laboratory studying the regulation of a newly isolated gene and the Biochemistry of a new transcription factor. The book reveals not only what to do but why and how to plan properly for success. The second edition updates material in the first edition, but more significantly, there has been a huge increase in the importance of chromatin-based mechanisms and protocols, almost absent in the first edition. It is to cover these that the third author, Craig Peterson, has been brought in.