Nanopore-Based Technology

Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality. In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization. Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing. Written for the highly successful Methods in Molecular Biology? series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results. Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths.

Ashok Agarwal is the Director of the Clinical Andrology Laboratory and Reproductive Tissue Bank, and the Director of Research at the Center for Reproductive Medicine since 1993. He holds these positions at The Cleveland Clinic Foundation, where he is a Professor at the Lerner College of Medicine of Case Western Reserve University. Ashok received his Ph.D. in 1983 and did his post-doctoral training in Reproductive Biology under a fellowship from The Rockefeller Foundation at Harvard Medical School, Boston. He was an Instructor in Surgery and then an Assistant Professor of Urology at Harvard Medical School from 1988 to 1993. Ashok has published over 500 scientific papers and reviews in peer reviewed scientific journals. He is currently an editor of 10 medical text books/ manuals related to male infertility, ART, fertility preservation, DNA damage and antioxidants. Ashok is active in basic and clinical research and his laboratory has trained over 150 basic scientists and clinical researchers from the United States and abroad. In addition, more than 150 medical, undergraduate, and high school students have worked in his laboratory. His current research interests include studies on molecular markers of oxidative stress, DNA integrity, and apoptosis in the pathophysiology of male and female reproduction, effect of radio frequency radiation on fertility and fertility preservation in patients with cancer.R. John?Aitken’s research career began with a PhD in reproductive biology from the University of Cambridge. Following post-doctoral positions at the Institute of Animal Genetics, University of Edinburgh and the University of Bordeaux, he joined the World Health Organization in Geneva, where he managed two independent WHO task forces dealing with different approaches to fertility regulation.? In 1977, he joined the Medical Research Council’s Reproductive Biology Unit, University of Edinburgh, to establish a research group in gamete biology with clinical outreach into male infertility. In 1992, John was awarded an Honorary Professorship within the Faculty of Medicine , University of Edinburgh, and in 1995 was elected a Fellow of the Royal Society of Edinburgh.? In 1998 he received an ScD degree from the University of Cambridge in recognition of his contribution to the field of gamete biology.? In the same year, he moved to the University of Newcastle, NSW, as Chair of Biological Sciences and, later, Director of the ARC Centre of Excellence in Biotechnology and Development. ?In 2011 he was elected to the Australian Academy of Science and is currently Laureate Professor of Biological Sciences and Director of the Priority Research Centre in Reproductive Science at the University of Newcastle. Professor Juan G. Alvarez received his Medical Doctor degree at the University of Santiago of Compostela in Spain, has a Ph.D in Physical-Chemistry by the University of Villanova, Pennsylvania, and was Associate Professor of Obstetrics and Gynecology and Reproductive Biology at Harvard Medical School. During over three decades he has developed his scientific career in world leading research centers such as the Reproductive Biology Unit at the University of Pennsylvania and Harvard Medical School, where he was Director of Research at the Department of Obstetrics and Gynecology and Co-Director of the Women’s Health Research Institut at Beth Israel Deaconess Medical Center in Boston. He was also Scientific Director of Institut Marques in Barcelona, one of the leading IVF centres in Spain. He carried out basic and clinical research in the field of infertility with particular emphasis in oxygen radical chemistry and biochemistry in mammalian spermatozoa and free radical-induced sperm DNA damage in human spermatozoa. Currently, he is Director of Centro ANDROGEN, one of the leading male infertility clinics in Spain. He combines his clinical activities with research in the area of male infertility. He collaborates with the Cleveland Clinic Glickman Urologic