Isolation and Purification of Proteins

This collection describes the various unit operations used for protein isolation and purification, and highlights the new tools and strategies that have been developed to make downstream processing more efficient, clean, and cost-effective. The 16 contributions discuss chromatography, genetic strategies for modifying recombinant proteins, and techniques commonly employed for removal of solids, volume reduction, and direct capture of the target protein from unclarified feedstock. Other topics include harvesting cells from the biological hosts, crystallization for large scale bioseparation, process monitoring during protein purification, and regulatory requirements for cleaning and sanitization. Annotation (c) Book News, Inc., Portland, OR (booknews.com)