Celiac Disease ― Methods and Protocols

Research gastroenterologists, immunologists, and other scientists detail laboratory methods for studying the biology and immunopathology of celiac disease, which involves sensitivity to gluten. They include reproducible techniques for cloning lymphocytes, using physical methods to identify gluten sequences as T-cell antigens, studying peptide responses in vitro and in vivo by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, and using elegant immuno-histo-cyto-chemical and mRNA probing techniques for analyzing the finer points of mucosal inflammatory response to gluten. Gluten sensitivity research is gaining popularity, and the descriptions here should allow newcomers to begin with the current methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)