Bioassays in Experimental and Preclinical Pharmacology
商品資訊
ISBN13：9781071612323
出版社：Humana Pr
作者：Karuppusamy Arunachalam
出版日：2021/01/23
裝訂：精裝
商品簡介
1. Overview
2. Cell Culture Assays
2.1 Cell Cycle Assay: Propidium Iodide Marking
2.2 Apoptosis Assay: Annexin V/Propidium Iodide Marking
2.3 Cytotoxicity in Caco-2 cells by the Alamar blue method
2.4 Viability in Caco-2 cells stimulated with lipopolysaccharide (LPS)
2.5 Determination of Nitric oxide (NO) by in vitro experiment
2.6 Analysis of specific cytotoxicity against RAW 264.7 for determination anti-inflammatory activity in vitro
2.7 Scratching of Fibroblast cells (scratch assay)
2.8 Evaluation of the in vitro inflammatory response in AGS cells
3. Phytochemical Analysis
3.1 Preliminary phytochemical assays
3.2 Quantitative analysis of secondary metabolites
3.3 Thin Layer Chromatography Analysis
3.4 Analysis by High Performance Liquid Chromatography (HPLC)
3.5 Mass spectrometry
3.6 Electrospray Ionization Mass Spectrometry (ESI-MS) analysis3.7 Identification of phenolic compounds by HPLC-LC-ESI- MS/MS
3.8 Quantitative determination of total alkaloids content by HPLC3.9 Quantitative determination of total alkaloids content by HPLC
4. Preclinical Drug Dose Calculation
4.1 Conversion based on normalization of dose-to body surface area
4.2 Determination of animal equivalent dose5. Toxicology Studies (In Vitro and In Vivo)
5.1 Cytotoxicity evaluation using Alamar blue5.2 Micronucleus test
5.3 Comet test5.4 Effect on cell cycle
5.5 In vivo acute toxicity study
5.6 Subchronic toxicity (repeated doses)
6. Standard Procedure for Anesthesia in Preclinical Experiments
6.1 Planning and anesthetic care
6.2 Pre-Procedure
6.3 Experimental Procedure
7. General Considerations and Collection of Animal Blood
7.1 Vein blood collection
7.2 Tail vein blood collection
7.3 Cardiac puncture blood collection
7.4 Posterior vena cava
7.5 Orbital sinus8. Animal Experiments on the Cardiovascular System
8.1 Obtaining electrocardiogram (ECG) and blood pressure (BP) signals8.2 Myocardial ischemia induction
9. Animal Experiments on Ulcerative Colitis
9.1 Ulcerative colitis induced by 2,4,6-Trinitrobenzenesulfonic acid (TNBS)
9.2 Chronic ulcerative colitis induced by Dextran Sodium Sulfate (SSD) 2%9.3 Colitis with recurrence (Chronic)
10. Experiments on Anti-Bacterial Activities
10.1 Antibacterial activity by in vitro assays
10.2 Animal Experiments on antibacterial activity11. Experiments on Anti-Fungal Activities
11.1 Agar Disc Diffusion Method11.2 Minimum Inhibitory Concentration (MIC)
11.3 Sorbital test11.4 Neurospora crassa assay
11.5 Effect of Ergosterol on MIC
11.6 Test of Β1,3-D-Glucan Synthase (GS)
11.7
今日66折
您曾經瀏覽過的商品
購物須知
外文書商品之書封，為出版社提供之樣本。實際出貨商品，以出版社所提供之現有版本為主。部份書籍，因出版社供應狀況特殊，匯率將依實際狀況做調整。
無庫存之商品，在您完成訂單程序之後，將以空運的方式為你下單調貨。為了縮短等待的時間，建議您將外文書與其他商品分開下單，以獲得最快的取貨速度，平均調貨時間為1~2個月。
為了保護您的權益，「三民網路書店」提供會員七日商品鑑賞期(收到商品為起始日)。
若要辦理退貨，請在商品鑑賞期內寄回，且商品必須是全新狀態與完整包裝(商品、附件、發票、隨貨贈品等)否則恕不接受退貨。