Notch Signaling Research: Methods and Protocols

1. Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster

Oleg V. Andreyenkov, Elena I. Volkova, Natalya G. Andreyenkova, and Sergey A. Demakov

2. Screening Mutants by Single Fly Genomic PCR

Usha Nagarajan and Marios Georgiou

3. Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation

Chenyu Ma, Yohei Tsukamoto, and Hideyuki Takeuchi

4. Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

5. High-Throughput Analysis to Identify Activators of Notch Signaling

Rachael Guenter, Jacob Eide, Herbert Chen, J. Bart Rose, and Renata Jaskula-Sztul

6. Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads

Takamasa Mizoguchi, Hikaru Handa, Shuhei Omaru, and Motoyuki Itoh

7. Mammalian NOTCH Receptors Activation and Signaling Protocols

Mar燰 Luisa Nueda and Victoriano Baladr鏮

8. Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation

Vartika Sharma, Nalani Sachan, Mousumi Mutsuddi, and Ashim Mukherjee

9. Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit

Kostiantyn Dreval, Robert J. Lake, and Hua-Ying Fan

10. Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters

Manoj Panta, Andrew J. Kump, Kristopher R. Schwab, and Shaad M. Ahmad

11. Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context

ChangHwan Lee, Tina Lynch, Sarah L. Crittenden, and Judith Kimble

12. An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers

Lily Paculis, Qiuping Xu, Qian Xie, and Dongyu Jia

13. Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster

Cordula Schulz

14. Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos

Elisabete Silva, Patr獳ia Diniz, Alexandre Trindade, Mariana Batista, Ana Torres, Ant鏮io Duarte, and Lu疄 Lopes-da-Costa

15. Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies

Laura M嫫quez-Exp鏀ito, Carolina Lavoz, Elena Cantero-Navarro, Ra R. Rodrigues-Diez, Sergio