Long Non-Coding RNAs in Cancer
商品資訊
ISBN13：9781071615836
出版社：Humana Pr
作者：Alfons Navarro(EDI)
出版日：2022/07/08
裝訂：平裝
規格：25.4cm*17.8cm*2.1cm (高/寬/厚)
商品簡介
PART I: LNCRNA CLASSIFICATION AND UTILITY IN CANCER
1. LncRNAs and Available Databases
Sara Napoli
2. LncRNA as Cancer Biomarkers
Ingram Iaccarino and Wolfram Klapper
PART II: LNCRNA PROFILING AND DISCOVERY
3. Bioinformatics Pipeline to Analyze lncRNA Arrays
Katia Todoerti, Domenica Ronchetti, Martina Manzoni, Elisa Taiana, Antonino Neri, and Luca Agnelli
4. Bioinformatic Pipelines to Analyze lncRNAs RNAseq Data
Luca Agnelli, Stefania Bortoluzzi, and Giancarlo Pruneri
5. Single Cell RNAseq Analysis of lncRNAs
Stefano Cagnin, Enrico Alessio, Raphael Severino Bonadio, and Gabriele Sales
PART III: VALIDATION OF IDENTIFIED LNCRNAS
6. Measuring lncRNA Expression by Real Time PCR
Sonam Dhamija and Manoj B. Menon
7. Quantitation of Long Non-coding RNA using Digital PCR
Yu Ota, Irene K Yan, and Tushar Patel
8. Detection of lncRNA by LNA-based In Situ Hybridization in Paraffin Embedded Cancer Cell Spheroids
Boye Schnack Nielsen, Jesper Larsen, Jakob H ffding, Son Ly Nhat, Natasha Helleberg Madsen, Trine M ller, Bj rn Holst, Kim Holmstr m
PART IV: FUNCTIONAL STUDIES TO DECIPHER THE ROLE OF IDENTIFIED LNCRNAS
9. In vitro Silencing of lncRNA Expression using siRNAs
Meike Thijssen, Jennifer Bintz, and Luis Arnes
10. In vitro silencing of lncRNAs using LNA gapmeRs
Elisa Taiana, Vanessa Favasuli, Domenica Ronchetti, Eugenio Morelli, Pierfrancesco Tassone, Giuseppe Viglietto, Nikhil C. Munshi, Antonino Neri, and Nicola Amodio
11. Methods used to make Lipid Nanoparticles to deliver LNA Gapmers against lncRNAs into Acute Myeloid Leukemia (AML) Blasts
Chun-Tien Kuo, Robert Lee, and Ramiro Garzon
12. CRISPR/Cas9 to Silence Long Non-coding RNAs
Ingrid Arctander Rosenlund, George A. Calin, Mihnea P. Dragomir, and Erik Knutsen
13. CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) to explore the oncogenic lncRNA network
Eugenio Morelli, Annamaria Gulla', Nicola Amodio, Elisa Taiana, Antonino Neri, Mariateresa Fulciniti, and Nikhil C. Munshi
14. In vivo Silencing/Overexpression of lncRNAs by CRISPR/Cas System
Marianna Vitiello, Laura Poliseno, and Pier Paolo Pandolfi
15. Experimental Validation of the Non-Coding Potential for lncRNAs
Emily Dangelmaier and Ashish Lal
16. Identification of lncRNA-protein interactions by CLIP and RNA pull-down assays
Kunming Zhao, Xingwen Wang, and Ying Hu
17. Empirical Validation of Overlapping Virus lncRNAs and Coding Transcripts by Northern Blot
Mehmet Kara and Scott A. Tibbetts
PART V: LNCRNA ANALYSIS IN LIQUID BIOPSY
18. Phospho-RNAseq profiling of extracellular mRNAs and lncRNAs
Maria D. Giraldez and Muneesh Tewari
19. Detection of Circulating RNA using Nanopore Sequencing
Jennifer Lindemann, Irene K Yan, and Tushar Patel
20. LncRNA Quantification from Extracellular Vesicles Isolated from Blood Plasma or Conditioned Media
Joan J. Castellano, Jordi Canals, Bing Han, Tania D燰z, Mariano Monzo, and Alfons Navarro
PART VI: CIRCULAR RNAS
21. The use of circRNAs as Biomarkers of CancerCarla Sol? Gartze Mentxaka, and Charles H. Lawrie
22. Bioinformatic Analysis of Circular RNA Expression
Enrico Gaffo, Alessia Buratin, Anna Dal Molin, and Stefania Bortoluzzi
23. Study of circular RNAs (circRNAs) expression
Eleonora D'Ambra and Mariangela Morlan
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