Therapeutic Antibodies: Methods and Protocols
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1. Therapeutic Antibodies: An Overview
Gunnar Houen
2. Treatment of Autoimmune Diseases with Therapeutic Antibodies: Lessons Learned from PID Patients Allow for Stratification of the Infection Risk
Joyce J.B.C. van Beers and Jan G.M.C. Damoiseaux
3. B-Cell Analysis for Monitoring Patients Undergoing B-Cell Depletion for the Treatment of Autoimmune Diseases
Jan G.M.C. Damoiseaux, Joyce van Beers, Matthias Busch, and Pieter van Paassen
4. Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods
Adriana-Michelle Wolf Perez, Nikolai Lorenzen, Michele Vendruscolo, and Pietro Sormanni
5. The Therapeutic Antibody Profiler for Computational Developability Assessment
Matthew I.J. Raybould and Charlotte M. Deane
6. Short Read-Length Next-Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs
Landon Pastushok, Kris Barreto, and C. Ronald Geyer
7. Large Scale Transient Production in ExpiCHO-STM with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection
Xiaotian Zhong, Ashley Schwab, Weijun Ma, Caryl L. Meade, Jing Zhou, Aaron M. D' Antona, Will Somers, and Laura Lin
8. Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris
Laia Montoliu-Gaya and Sandra Villegas
9. Purification of Therapeutic Antibodies by Protein A Affinity Chromatography
Anurag S. Rathore and Sharad Narnaware
10. Ion Exchange Chromatographic Methods for Purification of Therapeutic Antibodies
Anurag S. Rathore and Vishwanath Hebbi
11. Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies
Noriko Iwamoto and Takashi Shimada
12. Use of PASEF for Accelerated Protein Sequence Confirmation and De Novo Sequencing with High Data Quality
Detlev Suckau, Waltraud Evers, Eckhard Belau, Stuart Pengelley, Anja Resemann, Wilfred Tang, K. Ilker Sen, Elsa Wagner, Olivier Colas, and Alain Beck
13. Nano-Microscopy of Therapeutic Antibody Aggregates in Solution
Yukako Senga, Toshihiko Ogura, Hiroshi Imamura, and Shinya Honda
14. Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering
Fabian Dingfelder, Anette Henriksen, Per-Olof Wahlund, Paolo Arosio, and Nikolai Lorenzen
15. Intact Mass Quantitation of Therapeutic Antibodies for Pharmacokinetic Studies Using Immuno-Purification
Lisa A. Vasicek, Daniel S. Spellman, and Kevin P. Bateman
16. Detection of Residual Host
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