Archaea: Methods and Protocols
商品資訊
ISBN13：9781071624449
出版社：Humana Pr
作者：Sébastien Ferreira-Cerca(EDI)
出版日：2022/10/22
裝訂：精裝
規格：25.4cm*17.8cm*3.2cm (高/寬/厚)
商品簡介
1. Archaea: a goldmine for molecular biologists and evolutionists
Patrick Forterre
Part I: Genetic manipulation of Archaea
2. Progress and challenges in archaeal genetic manipulation
Catherine Harrison and Thorsten Allers
3. Genetic manipulation of Haloferax species
Ambika Dattani, Catherine Harrison, and Thorsten Allers
4. CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii
Thandi S. Schwarz, Sandra S. Schreiber, and Anita Marchfelder
5. Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis
Geraldy L. S. Liman, Meghan E. Stettler and Thomas J. Santangelo
6. Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea
Johanna Thomsen, Katrin Weidenbach, William W. Metcalf and Ruth A. Schmitz
7. An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ΔH
Christian Fink, Largus T. Angenen, and Bastian Molitor
8. Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius
Xing Ye, Alejandra Recalde, Sonja-Verena Albers and Marleen van Wolferen
9. A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus
Changyi Zhang, Serina M. Taluja, Emily N. Hallett, and Rachel J. Whitaker
10. Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus
Changyi Zhang and Rachel J. Whitaker
11. Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales
Erika Wimmer, Isabelle Anna Zink and Christa Schleper
Part II: Molecular Biology of Archaea
12. Progress and challenges in archaeal molecular biology
Finn Werner
13. ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery
Fabian Blombach, Kathy L. Smollett, and Finn Werner
14. Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii
Diego Rivera Gelsinger, Emma Dallon, and Jocelyne DiRuggiero
15. Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii
Diego Rivera Gelsinger and Jocelyne DiRuggiero
16. Quantitative Mass Spectrometry by SILAC in Haloferax volcanii - Ricardo L. Couto-Rodriguez, Daniel Gal, Lana J. McMillan, Jin Koh, Sixue Chen, and Julie A. Maupin-Furlow
17. Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach
Roberto A. Paggi, Stefan P. Albaum, Ansgar Poetsch, and Micaela Cerletti
18. Proteomic sample preparation and data analysis in line with the Archaeal Proteome
Stefan Schulze and Mechthild Pohlschroder.
19. In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii
Roberto A. Paggi, Rosana E. De Castro and Micaela Cerletti
20. Proteolytic activity assays in haloarchaea
Roberto Alejandro Paggi, Mar燰 In廥. Gim幯ez, and Rosana Esther De Castro
21. Carotenoids from Haloarchaea: Extraction, fractionation and characterization
Mar燰 Sandra Churio, Micaela Cerletti, and Rosana Esther De Castro
22. Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii Mariana In廥 Costa and Mar燰 In廥 Gim幯ez
23. Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-glucon
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