Lipopolysaccharide Transport: Methods and Protocols
商品資訊
ISBN13：9781071625804
出版社：Humana Pr
作者：Paola Sperandeo(EDI)
出版日：2022/10/26
裝訂：精裝
規格：25.4cm*17.8cm*1.8cm (高/寬/厚)
商品簡介
PART I Genetic Strategies to Study Essential LPS Biogenesis Genes
1. Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport
Andrew Wilson, Carlos Iniguez, and Natividad Ruiz
2. Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa
Alessandra Lo Sciuto, Maria Concetta Spinnato, Martina Pasqua, and Francesco Imperi
3. Analysing the Function of Essential Genes by Plasmid Shuffling
Federica Anna Falchi
PART II Functional Analysis of Proteins Involved in LPS Transport
4. Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia Coli
Elisabete C. Cardoso Mendes Moura, Alessandra Polissi, and Paola Sperandeo
5. Exploring The Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in silico and Biochemical Analyses
Julia Monjar嫳 Feria and Miguel A. Valvano
6. Use of Site‐Directed Spin Labeling EPR Spectroscopy to Study Protein-LPS Interactions
Kathryn M. Schultz and Candice S. Klug
7. A Photo-Crosslinking Approach to Monitor Assembly of an LptD Intermediate with LptE in a Living Cell
Ryoji Miyazaki, Hiroyuki Mori, and Yoshinori Akiyama
8. Mass Spectrometry Analysis of Dynamics and Interactions of the LPS translocon LptDE
Francesco Fiorentino and Jani R. Bolla
9. Affinity Purification and Co-Immunoprecipitation of Trans-envelope Protein Complexes in Gram-negative Bacteria
Alessandra M. Martorana, Carlo Santambrogio, and Alessandra Polissi
10. A Bacterial Two-Hybrid System for in vivo assays of Protein-Protein Interactions and Drug Discovery
Daniel Ladant
11. Detecting Lipoproteins Sneaking out of the Lipopolysaccharide Leaflet
Naemi Csoma, Didier Colau, and Jean-Fran蔞is Collet
PART III Tools for Structural Analysis of LPS and LPS Transport Proteins
12. Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry
Pilar Garcia-Vello, Immacolata Speciale, Flaviana Di Lorenzo, Antonio Molinaro, and Cristina De Castro
13. Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective
Karishma Biswas and Anirban Bhunia
14. Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA
Fran蔞is A. Th幨ot and Maofu Liao15. Protein Crystallization of Two Recombinant Lpt Proteins
Michela Bollati and Louise J. Gourlay
PART IV Microscopy-Based Approaches
16. Metabolic Incorporation of Azido-Sugars into LPS to Enable Live Cell Fluorescence Imaging
Inga Nilsson and David A. Six
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