Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Method
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital
In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Prot
Invasive fungal infections are a significant cause of morbidity and mortality. Over the past decade there has been a concerted effort to develop reliable methods for the detection of such infections.
Detailing current methodology, this book examines important analytical techniques in chiral separation sciences. It includes step-by-step, readily reproducible laboratory protocols and troubleshooting
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.? In Signal Transd
Oncological imaging has thoroughly changed in the past decade, especially due to the introduction of PET and 18FDG. In Positron Emission Tomography, expert referring specialists and professional image
The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.? In cDNA Libraries: Methods and Protocols, expert res
John R. Crowther provides todayA-s premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and P
The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein a
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell leve
This volume is valuable and timely resource for a broad audience with interests in basic and translational cancer biology, cancer drug development, as well as in the practice of personalized oncology.
This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of
This volume, the first of two, provides a set of tutorials for molecular biologists using comparative genomic analysis tools and, in particular, aims to make accessible not only the commonly used tech
International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteri
