Cellular in Vitro Testing ― Methods and Protocols
商品資訊
ISBN13:9789814364973
出版社:Taylor & Francis
作者:John Haycock (EDT); Arti Ahluwalia (EDT); J. Malcolm Wilkinson (EDT)
出版日:2014/05/21
裝訂/頁數:精裝/300頁
商品簡介
Focusing on new 3-D in vitro methods, this book brings together examples of leading-edge work being conducted internationally for improving in vitro cell culture methods. It highlights the use of systems for enabling cell culture under laminar flow and the use of 3-D scaffolds for providing cells with a structure which replicates the function of the extracellular matrix and encouraging interactions more akin to an in vivo environment. Chapters cover state-of-the-art protocols for bioreactor systems, in vitro toxicity methods, non-invasive imaging, 3-D cell cultures, stem cell usage, and the integration and use of novel scaffolds.
作者簡介
John Haycock was educated at Newcastle University U.K. (BSc Biochemisty, PhD Neuroscience) and after a PDRA in New York (USA) and Sheffield University (U.K.) became a lecturer at Sheffield University in 2001. He was promoted to Senior Lecturer (2005), Reader (2008) and presently holds a chair in Bioengineering (2011) at the Department of Material Science & Engineering and the Kroto Research Institute, University of Sheffield. He is also Director of the Centre for Biomaterials & Tissue Engineering and Associate Director of the Kroto Research Institute at Sheffield. Key areas of his work include scaffolds for nerve injury repair, 3D nerve models as alternatives for animal testing and 3D imaging. His publications include papers in Nature Protocols, Proc Natl Acad Sci, and Adv. Mater and he has edited 1 previous book entitled 3D Cell Culture published under the Methods in Molecular Biology (2011) series of texts (Humana Press / Springer Protocols, USA). Prof Haycock is a member of the EPSRC Peer Review College in the UK and internationally has serves on grant awarding panels and examination bodies in Canada (CFI), Finland and Sweden.
Arti Ahluwalia has a PhD in Bioengineering from the Polytechnic of Milan (IT), MSc. in Instrumentation and Analytical Science, University of Manchester (UMIST, UK), BSc. in Physics, University of Bath (UK) and is now Associate Professor of Bioengineering at the Department of Information Engineering, Faculty of Engineering, University of Pisa, and Vice Director of Interdepartmental Research Center "E. Piaggio", head of the MCB Group. Affiliated with National Council of Research Institute of Clinical Physiology (CNR-IFC) and director of its NanoBioScopy Lab. The main focus of her research has centered on the interaction between biological systems and man-made materials devices or structures focused towards the creation of organ and system models in-vitro. Author of over 100 peer-reviewed publications and of several patents on microfabrication, and on microfabricated multicomaprtment bioreactors that have been licensed for commercialization. Current projects are on tissue engineering, biosensing, robots for autism, bioreactors and in-vitro models.
Malcolm Wilkinson is the founder and Managing Director of Kirkstall Ltd. Prior to founding Kirkstall he had been managing a high technology consulting company in Cambridge UK following a career in high technology product development both in large corporations as well as start-ups. He has had senior roles in most important functions from R&D through to sales and marketing. In the consulting company he supported spinouts from Universities and raised over $15 million from Venture capital and regional development funds. He has a BA from Oxford University, MSc from Southampton and did his PhD research at Middlesex University. He is a Fellow of the Institute of Nanotechnology and has been a visiting Lecturer for FSRM, Neuchatel, Switzerland, on the subject of Micro and Nanotechnology in Biomedical Engineering for over 10 years.
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